Coding
SUMO-MTs

Part:BBa_K4385006

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-01)


Metallothionein

Small ubiquitin-related modifier (SUMO) is a member of the ubiquitin-related protein family. SUMO modulates protein function through covalent conjugation to lysine residues in a large number of proteins.In our project, it can increase the soluble expression of MT protein. MT is a special small-molecule metal-binding protein in organisms, which is widely distributed, rich in cysteine, has a conserved structure, and has a high affinity for metals. After connected to the SUMO, it can be used as a supplement for surface display to enhance the tolerance of engineered bacteria to various heavy metal ions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 101
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None